Description

Product name: Bet v 1-A – Betula verrucosa (European white birch) allergen 1-A

Product description: Recombinant Betula verrucosa (European white birch) allergen 1-A produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

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Description

Host species: Nicotiana benthamiana

Molecular weight: 18.6 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Birch trees (Betula), are found in the temperate climate zone of the northern hemisphere and release large amounts of pollen during spring, a major cause of allergic rhinitis in Europe¹. A 17 kDa protein called Bet v 1-A² is the major allergen of birch pollen³. It has been shown that in birch-rich areas of Scandinavia, >95% of birch pollen allergic patients have immunoglobulin E (IgE)-binding to Bet v 1⁴.  Three regions on the surface of the protein contain cross-reactive B-cell epitopes that provide structural basis for similar allergic symptoms when encountering pollens from related trees such as hazel and alder⁵.

Specification

Purity: 98 % as shown by SDS-PAGE

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Bet v 1-A.

Biological activity: Not tested.

Endotoxin: Not tested.

Storage

Formulation: Lyophilized from 50 mM HEPES pH7.5, 100 mM NaCl, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

References

1. ^{Caillaud D, Martin S, Segala C, et al. Effects of airborne birch pollen levels on clinical symptoms of seasonal allergic rhinoconjunctivitis. Int Arch Allergy Immunol. 163(1):43–50. (2014)}
2. ^{https://www.uniprot.org/uniprot/P15494}
3. ^{Ipsen H, Løwenstein H. Isolation and immunochemical characterization of the major allergen of birch pollen (Betula verrucosa) J Allergy Clin Immunol. 72(2):150–159. (1983)}
4. ^{Movérare R, Westritschnig K, Svensson M, et al. Different IgE reactivity profiles in birch pollen sensitive patients from six European populations revealed by recombinant allergens: an imprint of local sensitization. Int Arch Allergy Immunol. 128(4):325–335. (2002)}
5. ^{Gajhede M, Osmark P, Poulsen FM, et al. X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy. Nat Struct Biol. 3(12):1040–1045. (1996)}