Human Tumor necrosis factor alpha (TNFα, TNF-α, cachexin)

£99.00

Prices from £99 (50μg ) –  For larger quantities, please contact us.

 

Product name: Human Tumor necrosis factor alpha (TNFα, TNF-α, cachexin)

Product description: Recombinant Human Tumor necrosis factor alpha (TNFα, TNF-α) soluble form (P01375|77-233) produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

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Description

Product name: Human Tumor necrosis factor alpha (TNFα, TNF-α, cachexin)

Product description: Recombinant Human Tumor necrosis factor alpha (TNFα, TNF-α) soluble form (P01375|77-233) produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Description

Host species: Nicotiana benthamiana

Molecular weight: 19.1kDa (+ Glycosylation)

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Tumor necrosis factor alpha (TNFα)1 is a cytokine that primarily expressed on the plasma membrane of activated T-lymphocytes and macrophages, that plays a diverse role in a range of infectious, inflammatory and malignant conditions2. The membrane-bound form is proteolytically processed by SPPL2A or SPPL2B to produce a cytosolic (ICD1 and ICD2) form and a c-terminal secreted extracellular form (sold here)3. In healthy individuals, TNFα is usually undetectable but becomes elevated during infectious and inflammatory conditions4.

Specification
Purity: 97 % as shown by SDS-PAGE

Human Granulocyte-macrophage colony-stimulating factor (GM-CSF, CSF2)

Figure 1:
reducing 4-20% SDS-PAGE analysis of 6 µg TNFα.

Biological activity: Not tested.
Endotoxin: Not tested.

Storage
Formulation: Lyophilized from PBS pH7.4, 175 mM Trehalose.

Shipping: Recombinant proteins are provided as lyophilized powder which is shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from the date of receipt at -70℃.
It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

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References: 
1. https://www.uniprot.org/uniprot/P01375
2. Bradley JR. TNF-mediated inflammatory disease. J Pathol. 214(2):149-60. Review. (2008).
3. Friedmann E, Hauben E, Maylandt K, Schleeger S, Vreugde S, Lichtenthaler SF,Kuhn PH, Stauffer D, Rovelli G, Martoglio B. SPPL2a and SPPL2b promoteintramembrane proteolysis of TNFalpha in activated dendritic cells to trigger IL-12 production. 8(8):843-8. (2006).
4. Robak T, Gladalska A, Stepien H. The tumour necrosis factor family of receptors/ligands in the serum of patients with rheumatoid arthritis. Eur Cytokine Netw. 9(2): 145–154. (1998).