MERS-CoV N-protein (Antigen)

Description

Product name:
MERS-CoV N-protein (Antigen)

Product description:
Recombinant MERS-CoV N-protein (Nucleoprotein, His-Tag) from produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Description

Expression host species: Nicotiana benthamiana

Host strain: England-Qatar/2012 (UniProtKB -PK9N4V7 (NCAP_MERS1)¹

Molecular weight: 45.7 kDa

Tag: C-terminal 6His

Uses: For research use only. Examples include SDS-PAGE, Western Blot, functional assays.

Relevance: The MERS-CoV viral nucleocapsid protein serves multiple critical functions during the viral life cycle. This 46 kDa protein, composed of 413 amino acids². It binds and packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) structure and has a core role in virion assembly through its interactions with the membrane protein (M) and the viral genome. The protein is composed of an N-terminal RNA binding domain and a C-terminal multimerization domain³.

Specification

Purity: 80 % as shown by SDS-PAGE (Figure 1).

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg MERS-CoV N-protein.
Protein runs as a monomer. Lower band (approx. 20 kDa) is a truncated form containing C-terminal oligomerization domain.

Biological activity: N/A

Endotoxin: Not tested.

Concentration: 0.85 mg/ml prior to lyophilization (analyzed by A₂₈₀ measurement)

Aliquot size: 1 x 100 µg per tube

Storage

Formulation: Lyophilized from 20 mM HEPES, 300 mM NaCl, pH 7.5, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

Protein runs as a monomer. Lower band (approx. 20 kDa) is a truncated form containing C-terminal oligomerization domain.

Protein Sequence

MASPAAPRAVSFADNNDITNTNLSRGRGRNPKPRAAPNNTVSWYTGLTQHGKVPLTFPPGQGVPLNANSTPAQNAGYWRRQDRKINTGNGIKQLAPRWYFYYTGTGPEAALPFRAVKDGIVWVHEDGATDAPSTFGTRNPNNDSAIVTQFAPGTKLPKNFHIEGTGGNSQSSSRASSVSRNSSRSSSQGSRSGNSTRGTSPGPSGIGAVGGDLLYLDLLNRLQALESGKVKQSQPKVITKKDAAAAKNKMRHKRTSTKSFNMVQAFGLRGPGDLQGNFGDLQLNKLGTEDPRWPQIAELAPTASAFMGMSQFKLTHQNNDDHGNPVYFLRYSGAIKLDPKNPNYNKWLELLEQNIDAYKTFPKKEKKQKAPKEESTDQMSEPPKEHRVQGTQRTRTRPSVQPGPMIDVNTDHHHHHH

References

1. ^{https://www.uniprot.org/uniprot/K9N4V7}
2. ^{Aboagye JO, Yew CW, Ng OW, Monteil VM, Mirazimi A, Tan YJ. Overexpression of the nucleocapsid protein of Middle East respiratory syndrome coronavirus up-regulates CXCL10. Biosci Rep. 2018;38(5):BSR20181059.}
3. ^{Verheije MH, Hagemeijer MC, Ulasli M, Reggiori F, Rottier PJ, Masters PS, de Haan CA. The coronavirus nucleocapsid protein is dynamically associated with the replication-transcription complexes. J Virol. 2010 Nov;84(21):11575-9.}