Description

Product name: Phlp1 Allergen – Phleum pratense (Common timothy) allergen 1

Product description: Phlp1 Allergen – Recombinant Phleum pratense (Common timothy) allergen 1 produced in Nicotiana benthamiana by Agrobacterium-mediated transient expression.

Description

Host species: Nicotiana benthamiana

Molecular weight: 29.5 kDa

Tag: C-terminal His-tag

Uses: For in vitro research use only. Not for human in vivo or therapeutic use.

Relevance: Phleum pratense (Common timothy) Phl p 1 is the major allergen from grass pollen¹. This glycoprotein is a group 1 allergen with a molecular mass of approximately 30 kDa².  Phl p 1 contains the majority of group 1–specific IgE and T cell epitopes³, and its homodimeric three-dimensional structure has been shown by x-ray crystallography. Phl p 1 is highly allergenic and can be used as a diagnostic marker to identify patients that are allergic to grass pollen due to its strong cross-reactivity with group 1 allergens from related grass species^4,5.

Specification

Purity: 96 % as shown by SDS-PAGE

Figure 1: reducing 4-20% SDS-PAGE analysis of 5 µg Phl p 1.

Biological activity: Not tested.

Endotoxin: Not tested.

Storage

Formulation: Lyophilized from 50 mM HEPES pH7.5, 100 mM NaCl, 175 mM Trehalose Dihydrate.

Shipping: Recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.

Stability & Storage: See COA for detailed storage instructions. Lyophilized materials are stable for up to twelve months from date of receipt at -70℃.

It is recommended that reconstituted protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution: A hardcopy of COA with reconstitution instructions is included with the product.

Contact us for more information.

References

1. ^{Laffer S, Valenta R, Vrtala S, Susani M, van Ree R, Kraft D, Scheiner O, Duchêne M. Complementary DNA cloning of the major allergen Phl p I from timothy grass (Phleum pratense); recombinant Phl p I inhibits IgE binding to group I allergens from eight different grass species. J. Allergy Clin. Immunol. 94:689–698. (1994).}
2. ^{https://www.uniprot.org/uniprot/P43213}
3. ^{Laffer S, Duchene M, Reimitzer I, Susani M, Mannhalter C, Kraft D, Valenta R. Common IgE-epitopes of recombinant Phl p I, the major timothy grass pollen allergen and natural group I grass pollen isoallergens. Mol. Immunol. 33:417–426. (1996).}
4. ^{Bokanovic D, Aberer W, Hemmer W, Heinemann A, Komericki P, Scheffel J, Sturm GJ. Determination of sIgE to rPhl p 1 is sufficient to diagnose grass pollen allergy. Allergy. 68:1403–1409. (2013)}
5. ^{Kahn CR, Marsh DG. Monoclonal antibodies to the major Lolium perenne (rye grass) pollen allergen Lol p I (Rye I) Mol. Immunol. 23:1281–1288. (1986)}